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A pinboard by
Danielle Tufts

Postdoc, Columbia University

PINBOARD SUMMARY

The first report of vertical transmission of Babesia microti in two wild host species.

I plan to present research of how Babesia (the causative agent of human babesiosis, which produces similar symptoms as malaria), is transmitted from a mother to her fetuses in the womb. This is the first time this process has been reported in the United States in the pathogen's natural hosts in the wild. Previously, the most commonly known transmission pathway was from ticks (Ixodes scapularis) to host (small rodents or humans), this research provides insights into other infection pathways for the spread of this disease into other regions and populations including humans.

4 ITEMS PINNED

Prevalence, genetic identity and vertical transmission of Babesia microti in three naturally infected species of vole, Microtus spp. (Cricetidae).

Abstract: Vertical transmission is one of the transmission routes for Babesia microti, the causative agent of the zoonotic disease, babesiosis. Congenital Babesia invasions have been recorded in laboratory mice, dogs and humans. The aim of our study was to determine if vertical transmission of B. microti occurs in naturally-infected reservoir hosts of the genus Microtus.We sampled 124 common voles, Microtus arvalis; 76 root voles, M. oeconomus and 17 field voles, M. agrestis. In total, 113 embryos were isolated from 20 pregnant females. Another 11 pregnant females were kept in the animal house at the field station in Urwitałt until they had given birth and weaned their pups (n = 62). Blood smears and/or PCR targeting the 550 bp 18S rRNA gene fragment were used for the detection of B. microti. Selected PCR products, including isolates from females/dams and their embryos/pups, were sequenced.Positive PCR reactions were obtained for 41% (89/217) of the wild-caught voles. The highest prevalence of B. microti was recorded in M. arvalis (56/124; 45.2%), then in M. oeconomus (30/76; 39.5%) and the lowest in M. agrestis (3/17; 17.7%). Babesia microti DNA was detected in 61.4% (27/44) of pregnant females. Vertical transmission was confirmed in 81% (61/75) of the embryos recovered from Babesia-positive wild-caught pregnant females. The DNA of B. microti was detected in the hearts, lungs and livers of embryos from 98% of M. arvalis, 46% of M. oeconomus and 0% of M. agrestis embryos from Babesia-positive females. Of the pups born in captivity, 90% were born to Babesia-positive dams. Babesia microti DNA was detected in 70% (35/50) of M. arvalis and 83% (5/6) of M. oeconomus pups. Congenitally acquired infections had no impact on the survival of pups over a 3-week period post partum. Among 97 B. microti sequences, two genotypes were found. The IRU1 genotype (Jena-like) was dominant in wild-caught voles (49/53; 92%), pregnant females (9/11; 82%) and dams (3/5; 60%). The IRU2 genotype (Munich-like) was dominant among B. microti positive embryos (20/27; 74%) and pups (12/17; 71%).A high rate of vertical transmission of the two main rodent genotypes of B. microti was confirmed in two species of naturally infected voles, M. arvalis and M. oeconomus.

Pub.: 09 Feb '17, Pinned: 05 Oct '17