Postdoc, Brown University
Our approach merges the ability of nanopores to force polymers to translocate in a linear fashion with the ability of mass spectrometry to identify individual amino acids.
Abstract: The passage of individual molecules through nanosized pores in membranes is central to many processes in biology. Previously, experiments have been restricted to naturally occurring nanopores, but advances in technology now allow artificial solid-state nanopores to be fabricated in insulating membranes. By monitoring ion currents and forces as molecules pass through a solid-state nanopore, it is possible to investigate a wide range of phenomena involving DNA, RNA and proteins. The solid-state nanopore proves to be a surprisingly versatile new single-molecule tool for biophysics and biotechnology.
Pub.: 26 Jul '08, Pinned: 17 Aug '17