ASSISTANT PROFESSOR, QUAID-I-AZAM UNIVERSITY, ISLAMABAD, PAKISTAN
My current research focuses on the involvement of Sirt family proteins in cutaneous wound healing and skin cell regeneration. My current research also focuses on the possible interaction of Sirtuins with other proteins as well as potential natural and synthetic Sirtuin regulators that can ameliorate the process of wound healing and induce rejuvenation and regeneration in the skin cells
Abstract: Skin cell regeneration and wound healing are key processes in the recovery from skin injuries. Rapid cell migration and regeneration of skin cells lead to faster and better healing of wounded skin. In the present study, we aimed to investigate the wound healing potential of juglone, a naturally occurring Pin1 inhibitor found in walnuts. Cultured skin cells (NHDF and HaCaT) and hairless mice were treated with juglone after wound creation to examine its effects on cell migration and wound healing rate. The expressions of cell migration related proteins (Rac1, Cdc42 and α-PAK), collagen deposition and angiogenesis were analyzed. Juglone treatment resulted in faster rate of growth and migration and recovered cell morphology, particularly at a concentration of 5 µM, in skin cells compared to the untreated group. In vivo experiments showed that mice treated with juglone showed faster wound healing rate with better skin morphology and collagen deposition than the vehicle group. Furthermore, juglone increased the activation and/or expression of Cdc42, Rac1, and α-pak in HaCaT cells, and resulted in enhanced angiogenesis in endothelial cells (HUVECs). Juglone also activated MAPKs signaling by activation of ERK, JNK and p38 proteins. Taken together, these data suggest that juglone may be a potential candidate for wound healing and skin regeneration which ameliorates wound healing mainly by promoting skin cell migration through Rac1/Cdc42/PAK pathway. This article is protected by copyright. All rights reserved.
Pub.: 11 Jun '16, Pinned: 19 Oct '17
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