A pinboard by
Nader Abu Farha

MD ( Medical Doctor ) Student, An Najah National University & An Najah National University Teaching Hospital


To investigate relatives’ attitudes towards informing patients with Alzheimer about their disgnosis

Alzheimer's disease ( AD ) is a chronic neurodegenerative disease that destroys memory and other important mental functions. It starts slowly with mild confusion and difficulty remembering, then it gets worse over time as the patients may forget important people in their lives and undergo dramatic personality changes. Alzheimer's disease is the most common cause of dementia, accounts for 60 - 80 % of all cases of dementia - a group of brain disorders that cause loss of intellectual and social skills. Because there's no cure for Alzheimer's disease, it's important to seek supportive services and support networks as early as possible.

Objectives : To evaluate & investigate relatives’ attitudes towards informing patients with Alzheimer’s disease (AD) about their diagnosis.

Setting : An Najah National University Teaching Hospital & Al-Watani Hospital in Palestine

Methods : The closest relatives of each of 61 subjects diagnosed for the first time as having AD will be interviewed, using a semistructured questionnaire. Spontaneous requests by the relatives to not inform the patients concerning the diagnosis will be also recorded. Why relatives choose to not fully inform the patient about his/her current condition and the degree of relationship between the patient and his/her caregiver will also be evaluated. In AD patients, dementia severity is measured using Global Deterioration Scale (GDS) and Minimental State Examination (MMSE) Before having the interview, AD clinical course is described with respect to progressive deterioration of mental functions and irreversibility of the process. The interview is based on specific questions concerning the following issues :

  • Diagnosis in terms of Alzheimer’s disease, dementia, or progressive memory loss
  • Poor prognosis, which is loss of all daily living activities within 10 years
  • Description of possible disease features
  • No disease modifying treatments

The UL8 subunit of the helicase/primase complex of herpes simplex virus promotes DNA annealing and has a high affinity for replication forks.

Abstract: During lytic infection, herpes simplex virus (HSV) DNA is replicated by a mechanism involving DNA recombination. For instance, replication of the HSV-1 genome produces X- and Y-branched structures, reminiscent of recombination intermediates. HSV-1's replication machinery includes a trimeric helicase/primase is composed of helicase (UL5) and primase (UL52) subunits and a third subunit, UL8. UL8 has been reported to stimulate the helicase and primase activities of the complex in the presence of ICP8, an HSV-1 protein that functions as an annealase, a protein that binds complementary single-stranded (ss)DNA and facilitates its annealing to duplex DNA. UL8 also influences the intracellular localization of the UL5/UL52 subunits, but UL8`s catalytic activities are not known. In this study, we used a combination of biochemical techniques and transmission electron microscopy. First, we report that UL8 alone forms protein filaments in solution. Moreover, we also found that, UL8 binds to ssDNAs >50 nt long and promotes the annealing of complementary ssDNA to generate highly branched duplex DNA structures. Finally, UL8 has a very high affinity for replication fork structures containing a gap in the lagging strand as short as 15 nt, suggesting that UL8 may aid in directing or loading the trimeric complex onto a replication fork. The properties of UL8 uncovered here suggest that UL8 may be involved in the generation of the X- and Y-branched structures that are the hallmarks of HSV replication.

Pub.: 27 Jul '17, Pinned: 31 Jul '17