NATIONAL POST DOCTORAL FELLOW, UNIVERSITY OF ALLAHABAD
PHYTOCHEMICAL ANALYSIS AND ANTI-HIV-1 ACTIVITY
Carissa carandas(family – Apocynaceae) is commonly known as Karonda in India. It is a nutritious wild fruit plant. The distribution is recorded throughout India mainly in the semi-arid regions. Since long time this plant has been used in traditional and folk medicine system. The fruits of this plant are little bit sour in taste but rich in vitamin C and iron contents. Fruits are used as astringent, appetizer, antipyretic, lessen-thirst, and biliousness and useful for the treatment of various diseases associated to brain. It is also used for treatment of indigestion, sexual dysfunction, and wound healing etc. Various extracts of different parts of this plant have been recorded for their various biological and pharmacological properties such as antioxidant, antidiabetic, anti-inflammatory anti-pyretic activity and wound healing etc. Fruit extracts of C. carandas were used for the different phytochemical analysis and antioxidant activity through various assays. Ethanolic, methanolic, hexane and aqueous extracts were used for this study. Different classes of phytoconstituents such as alkaloids, terpenes, fats and fixed oil, steroids, glycosides, cardiac glycosides, tannins were significantly present in various extracts. UV-Vis spectrophotometric analysis showed the presence of significant amount of phenolics and flavonoids. Total antioxidant activity was highest in aqueous extract. Maximum reducing potential was observed in methanolic extract. HPLC analysis of fruit extract showed the presence of various phenolic acids. Total phenolic content was highest in aqueous extracts. In-silico analysis of Carandinol, Carrisone, Carindoen, Gallic acid, Lupeol, and p-coumaric acid showed significant anti-HIV-1 activity against reverese transcriptase (1REV) and protease (1EBY). These findings suggest that this plant has immense potential to be used for the production of nutraceuticals, pharmaceuticals, and pharmacophores towards treatment of different ailments.
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