PhD student, Amity Institute of Biotechnology, Amity University Uttar Pradesh, India
In plant life cycle, leaf senescence is essential phenomenon which includes the degradation of macromolecules and redistribution of nutrients to the growing parts in extremely systematic manner. During leaf senescence, plant undergoes into stress conditions that inhibits carbon fixation due to which carbon dioxide availability reduces in leaves, that causes the enhanced production of ROS and extreme reduction of photosynthetic electron transport system in chloroplast that leads the yellowing of leaves and cell death. We report here the functional analysis of chloroplastic/mitochondrial GABA transaminase (GABA-T) of Arabidopsis thaliana, which plays a prominent role in regulation of carbon and nitrogen during leaf senescence and plant development process. Therefore, to investigate the function of GABA-T in leaf senescence, we have characterized GABA transaminase knock out mutants (pop2-1 and pop2-3) of Arabidopsis thaliana, which was accompanied by the assays of physiological parameters of leaf senescence conditions and GABA shunt components. Knowledge of the complete mechanism of this gene will help to delay the leaf senescence which will ultimately result in overall production in crops.