Ph.D student, Instituto Politecnico Nacional
The study evaluated the role of Stenotrophomonas maltophilia in the degradation of hydrocarbon
Stenotrophomonas maltophilia is known to be a very versatile group of gram-negative bacteria with different metabolic potentials among which is their potential to degrade xenobiotics, poly aromatic compounds, and organophosphates (Ryan, 2009). We isolated the five Stenotrophomonas maltophilia strains in this study from Oil contaminates sites in some part of Mexico. We tested their tolerance to and the ability to degrade different Poly Aromatic Hydrocarbon (Biphenyl, Phenanthrene, Phenanthridine, Naphthalene and Anthraquinone and Anthracene) as described by Singh et al., (2005) and Dans and Chandran, (2010) All isolated Stenotrophomonas maltophilia strains could tolerate 1% and 5% of 100mg/ml (≈ 1mg/ml and 5mg/ml) of five of the hydrocarbon tested ((Biphenyl, Phenanthrene, Phenanthridine, Naphthalene and Anthraquinone) and 1% and 5% of 40mg/ml (≈ 400μg/ml and 2mg/ml) of Anthracene)) These showed that all strains have tendency to use hydrocarbon as carbon source at the evaluated concentration and potential to degrade them. Their potentials for degrading hydrocarbon due to their ability to emulsify oil was evaluated as described by Dans and Chandran,
Abstract: In this study, two strains of Bdellovibrio were isolated from soil samples using the culture‐dependent technique and two members of the family Enterobacteriaceae (Klebsiella sp. and Salmonella sp.) as prey. The Bdellovibrio strains were bacteriolytic, plaque‐forming, and highly motile gram‐negative bacteria. We identified and confirmed the Bdellovibrio strains using microscopy, PCR amplification, and sequencing of the 16S rRNA gene. They were observed to be different strains based on hit locus and prey range analyses. Here, the first report on Bdellovibrio strains isolated from soil in Mexico corroborates earlier report indicating that populations of Bdellovibrio found in soil are heterogeneous thereby the need to identify the various strains.
Pub.: 13 Jun '16, Pinned: 30 Oct '17
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