A pinboard by
Temidayo Elufisan

Ph.D student, Instituto Politecnico Nacional


The study evaluated the role of Stenotrophomonas maltophilia in the degradation of hydrocarbon

Stenotrophomonas maltophilia is known to be a very versatile group of gram-negative bacteria with different metabolic potentials among which is their potential to degrade xenobiotics, poly aromatic compounds, and organophosphates (Ryan, 2009). We isolated the five Stenotrophomonas maltophilia strains in this study from Oil contaminates sites in some part of Mexico. We tested their tolerance to and the ability to degrade different Poly Aromatic Hydrocarbon (Biphenyl, Phenanthrene, Phenanthridine, Naphthalene and Anthraquinone and Anthracene) as described by Singh et al., (2005) and Dans and Chandran, (2010) All isolated Stenotrophomonas maltophilia strains could tolerate 1% and 5% of 100mg/ml (≈ 1mg/ml and 5mg/ml) of five of the hydrocarbon tested ((Biphenyl, Phenanthrene, Phenanthridine, Naphthalene and Anthraquinone) and 1% and 5% of 40mg/ml (≈ 400μg/ml and 2mg/ml) of Anthracene)) These showed that all strains have tendency to use hydrocarbon as carbon source at the evaluated concentration and potential to degrade them. Their potentials for degrading hydrocarbon due to their ability to emulsify oil was evaluated as described by Dans and Chandran,

  1. We noted that four (ASS1, ASS2, SVIA1, SVIA2) out of the five strains have potential to emulsify oil with strain SVIA2 showing the best emulsion stability. Owing to its inability to emulsify Hydrocarbon, the ability of Stenotrophomonas maltophilia strain Pemsol to degrade hydrocarbon was evaluated in a degradation study and the resultant compound following degradation was assessed using both quantitative and qualitative approach. The quantitative analysis using FTIR spectrometry following 15th and 30th days of incubation showed that bacteria have the potential to degrade Naphthalene. The degradation of Naphthalene by Stenotrophomonas maltophilia Pemsol follow the catechol dioxygenase pathway as indicated by the introduction of the hydroxyl group to the Naphthalene structure and the presence of several carbonyl and hydroxyl group on the Naphthalene structure after 30days. This quantitative analysis, therefore, showed that Stenotrophomonas maltophilia strain Pemsol could degrade Naphthalene though it does not display an emulsifying property. This study revealed the possibility of applying Stenotrophomonas maltophilia for the degradation of hydrocarbon.