PhD Student in Molecular Biology and Biotechnology, PAN AFRICAN UNIVERSITY OF BASIC SCIENCES, TECHNOLOGY AND INNOVATION
The aim of the study is to increase milk yield and improve fatty acids profiles in indigenous cows
In Benin, the main indigenous cows are White Fulani and Borgou cattle breeds. These breeds are characterized by low milk yield and there is no data available on their milk fatty acids composition. The trypanosomiasis is the main disease challenging milk production in Benin.The aim of my study the effects of the main milk proteins (CSN3, MBLG and LALBA) and milk fat (DGAT1 and SCD1) genes on milk yield and fatty acids composition of indigenous Borgou and White Fulani breeds and to assess the influence of trypanosomiasis disease on milk production. We found that CSN3 variants (SNps) were associated with high milk yield while DGAT1 and SCD1 genetic variants are associated with oleic and linoleic fatty acids which are important for human health. The specific alleles associated with high milk yield and important fatty acids will serve as genetic markers to increase milk yield and improve the fatty acids profiles of milk from indigenous cows from Benin. However, the work on trypanosomiasis is still ongoing.
Abstract: Polymorphisms located in the genes ABCG2, DGAT1, LEP, PRLR, RORC, CAPN1 and CAST previously have been associated with milk or meat production traits. In this study, these polymorphisms were examined for significant effects on reproductive traits [age at puberty (AGECL), post-partum anoestrus interval (PPAI) and the ability ovulate prior to weaning (PW)] and on a panel of correlated traits such as weight, growth and serum concentration of insulin-like growth factor I. The effects of the polymorphisms were examined in two samples of tropically adapted beef cattle: Brahman (N = 932) and Tropical Composites (N = 1097). A polymorphism in the gene DGAT1 was associated with age at puberty in the combined sample (P = 0.042), and two polymorphisms in CAPN1 were associated with PPAI (P = 0.033) and with the ability ovulate PW (P = 0.017). The favourable allele for reproductive traits was not always the favourable allele associated with production traits. The effects of these polymorphisms on reproductive traits were small compared to their effects on the traits for which they were originally discovered.
Pub.: 14 Apr '12, Pinned: 05 Dec '17
Abstract: Multi-copy gene families are especially prevalent in the male-specific region (MSY) of the mammalian Y chromosome. Copy number variations (CNV) of these Y-linked gene families have been shown to affect human and animal fertility. The PRAMEY (Preferentially expressed antigen in melanoma, Y-linked) gene family is a newly identified, bovid-specific Y-linked gene family, which codes for a cancer/testis antigen that is expressed predominantly in testis and various tumors. The PRAMEY gene family is believed to play an important role in spermatogenesis and male fertility in cattle. The objective of this study was to investigate the CNV of PRAMEY within and across breeds and to determine whether CNV was associated with reproductive traits in Holstein bulls. A quantitative real-time PCR method was applied to measure the copy number of PRAMEY among 460 bulls using a Y-linked single copy gene, DDX3Y (DEAD box polypeptide 3, Y-linked), as a reference. The median copy number of PRAMEY was 13, ranging from 2 to 31. Significant variations in PRAMEY copy number were observed among 15 breeds investigated. Holstein bulls had the lowest median copy number (12), whereas Limousin bulls possessed the highest median copy number (26). Furthermore, bulls in the taurine lineage (13) had a significantly lower median copy number than those bulls in the indicine lineage (20). Association analysis revealed that PRAMEY copy number was correlated negatively with scrotal circumference (SC), relative scrotal circumference (RLSC), percentage of normal sperm (PNS), and nonreturn rate (NRR), but had no significant association with postthaw motility (PTM), incubated motility (IM), percentage of intact acrosome (PIA), sire conception rate (SCR), or relative breeding efficiency (RBE). The data from this study indicate that CNV of the PRAMEY gene family is associated with male reproductive traits and may serve as a valuable marker for sire fertility selection at an early age in cattle.
Pub.: 15 Oct '13, Pinned: 05 Dec '17