Lecturer, University of Sargodha
Because of increasing side effects of synthetic medicines, the scientific research is being diverted toward the discovery of some better alternatives in pharmaceutical preparations from natural sources. The aim of current research work was to study the effect of Mucuna pruriens (MP) seed extract on liver enzymes, serum proteins and reproducive hormone levels in male albino rats. Seeds extract of M. pruriens was prepared in methanolic solvent and the potential effect was evaluated by administering into male albino rats for 42 days. The rats were divided into six groups of four rats in each group; as control (normal saline), Carbon tetrachloride injected (0.5 mL 20% CCl4 /Kg bw twice a week), Testosterone treated (0.5 mL 20% CCl4 + 2.5 mg testoverone/Kg bw twice a week) and MP-treated A, B & C (0.5 mL 20% CCl4 twice a week + 50, 100 & 200 mg extract/Kg bw daily, respectively) groups. Body, testis and relative testis weight of male rats were recorded. Liver enzymes and serum proteins were measured by photometric method using commercially available assay kits. Serum testosterone was determined by ELISA kit method. Histological changes in liver and testicular sections were observed through microscopy by H & E staining. Significant (p<0.05) increase in body, testis and relative testis weight was observed in extract treated rats compared to normal and CCl4 intoxicated rats. Significant (p<0.05) dose dependent improvement in ALT and AST activity was observed extract treated rats while not in ALP activity compared to control and CCl4 treated rats. Serum proteins particularly albumin concentration vary significantly (p<0.05) in extract treated rats. Significant (p<0.05) increase in serum testosterone level was also found in rats treated with testosterone and Mucuna seeds extract. Serious damage to liver cells (hepatocytes) and testicular histology in the CCl4 intoxicated rats was observed, while an improvement was found in testosterone and extract treated rats. The study concluded that MP seeds extract have therapeutic potential to revert the toxic effect of CCl4 bringing the biochemical and histological alterations toward normal. MP seeds extract may provide potential therapeutic agents to be used as medicines for various ailments.
Abstract: Infertility is defined as inability of a sexually active couple to conceive after 1 year of regular intercourse without contraception. Male factors account for 20%-50% of cases of infertility. The aim of this study was to review medicinal plants that proposed to improve sperm abnormalities in traditional Persian medicine. For this purpose, PubMed, Scopus, GoogleScholar and Cochrane library were explored for medicinal plants used in traditional Persian medicine for sperm abnormalities to obtain studies giving any evidence for their efficacy and pharmacological mechanisms related to male infertility. Data were collected for the years 1966 to March 2015. For some of them, including Chlorophytum borivilianum, Crocus sativus, Nigella sativa, Sesamum indicum, Tribulus terrestris, Mucuna pruriens and Withania somnifera, more reliable evidence was found. The mechanisms involved in the beneficial effects of medicinal plants in sperm abnormalities are antioxidant, anti-inflammatory, anti-oedematous and venotonic activity as well as containing precursors for sperm production and increasing blood testosterone level. Various phytochemical categories including saponins, phytosterols, carotenoids, oxygenated volatile compounds, phenolic compounds and alkaloids seem to be responsible for these beneficial effects. Further studies are recommended for obtaining more conclusive results about the efficacy and safety of the mentioned medicinal plants.
Pub.: 30 Sep '16, Pinned: 13 Nov '17
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