A pinboard by
Aniruddha Kaushik

Ph.D. Candidate, Johns Hopkins University


Microfluidic platforms for rapid and sensitive detection of infectious pathogens in human samples

Conventional methods used in hospital labs for identifying, counting, and characterizing drug resistance of bacteria that exist in infected human samples are slow and cumbersome, and can take as long as 2 days for definitive diagnosis. This can inevitably delay treatment of the infection, which may result in grave clinical outcomes. As a solution to this critical problem, I am developing a medical diagnostic device that can be used to trap individual bacterial cells in microscopic compartments known as "droplets." Once trapped, these bacteria can be identified and counted, based on their specific nucleic acid signatures. Moreover, the bacterial cells can be characterized as sensitive or resistant, based on their response to antibiotics. By relying on measurements from single-cells, definitive diagnosis can be achieved in as little as 1 to 2 hours, much faster than conventional methods. A diagnostic device such as this has the potential to improve patient outcomes as well as curtail the emergence and spread of drug resistant pathogens.


New and developing diagnostic technologies for urinary tract infections.

Abstract: Timely and accurate identification and determination of the antimicrobial susceptibility of uropathogens is central to the management of UTIs. Urine dipsticks are fast and amenable to point-of-care testing, but do not have adequate diagnostic accuracy or provide microbiological diagnosis. Urine culture with antimicrobial susceptibility testing takes 2-3 days and requires a clinical laboratory. The common use of empirical antibiotics has contributed to the rise of multidrug-resistant organisms, reducing treatment options and increasing costs. In addition to improved antimicrobial stewardship and the development of new antimicrobials, novel diagnostics are needed for timely microbial identification and determination of antimicrobial susceptibilities. New diagnostic platforms, including nucleic acid tests and mass spectrometry, have been approved for clinical use and have improved the speed and accuracy of pathogen identification from primary cultures. Optimization for direct urine testing would reduce the time to diagnosis, yet these technologies do not provide comprehensive information on antimicrobial susceptibility. Emerging technologies including biosensors, microfluidics, and other integrated platforms could improve UTI diagnosis via direct pathogen detection from urine samples, rapid antimicrobial susceptibility testing, and point-of-care testing. Successful development and implementation of these technologies has the potential to usher in an era of precision medicine to improve patient care and public health.

Pub.: 02 Mar '17, Pinned: 19 Sep '17