A pinboard by
Xiaodong Liu

PhD student, Monash University


Pluripotency is an important feature of human embryonic stem cells and human induced pluripotency stem cells. Recent reports of the exact characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a broad spectrum of naive characteristics. Furthermore, our characterisation identifies KLF4 as a conduit for conversion of primed hPSCs into naive t2iLGöY hPSCs underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.