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CURATOR
A pinboard by
RITIKA DWIVEDI

Postdoctoral Associate, Broad Institute of MIT and Harvard

PINBOARD SUMMARY

Use of high throughput technologies to remove bottlenecks in drug design

Currently, major limitations exist to oral delivery of peptide based drugs, due to decreased stability in the digestive tract. We are generating libraries of stable therapeutics by using protein engineering within stable frameworks to tackle this problem. We have generated several promising candidates for orally available drugs for diabetes.

5 ITEMS PINNED

Understanding the Diversity and Distribution of Cyclotides from Plants of Varied Genetic Origin.

Abstract: Cyclotides are a large family of naturally occurring plant-derived macrocyclic cystine-knot peptides, with more than 400 having been identified in species from the Violaceae, Rubiaceae, Cucurbitaceae, Fabaceae, and Solanaceae families. Nevertheless, their specialized distribution within the plant kingdom remains poorly understood. In this study, the diversity of cyclotides was explored through the screening of 197 plants belonging to 43 different families. In total, 28 cyclotides were sequenced from 15 plant species, one of which belonged to the Rubiaceae and 14 to the Violaceae. Every Violaceae species screened contained cyclotides, but they were only sparsely represented in Rubiaceae and nonexistent in other families. The study thus supports the hypothesis that cyclotides are ubiquitous in the Violaceae, and it adds to the list of plants found to express kalata S and cycloviolacin O12. Finally, previous studies suggested the existence of cyclotide isoforms with either an Asn or an Asp at the C-terminal processing site of the cyclotide domain within the precursor proteins. Here we found that despite the discovery of a few cyclotides genuinely containing an Asp in loop 6 as evidenced by gene sequencing, deamidation of Asn during enzymatic digestion resulted in the artifactual presence of Asp isoforms. This result is consistent with studies suggesting that peptides can undergo deamidation after being subjected to external factors, including pH, temperature, and enzymatic digestion.

Pub.: 05 May '17, Pinned: 29 Jun '17