Ph.D, Faculty of Medicine of Tunis
Efficacity of Ailanthus altissima on pulmonary fibrosis in rat
The present study aimed to investigate the protective effect of Ailanthus altissima wood aqueous extract (Awe) against bleomycin (BLM)-induced lung fibrosis as well as the involvement of oxidative stress in such protection. In this respect, adult male wistar rats were used and divided into three groups of twenty each: control (NaCl, 0.9%), BLM and BLM (4 mg/kg b.w.) + Awe (200 mg/kg, b.w.). The rat model of pulmonary fibrosis (PF) was established by intratracheal instillation of BLM, and the effect of 200 mg/kg, b.w Awe treatment once daily observed. The effect of the treatment was studied over a period of 30 days using 1H RMN analysis on the broncho alveolar lavage fluid (Balf) of the rats. Histopathological (inflammation and fibrosis) and immunohistochemical (TGF-β1 density) changes were evaluated. Administration of BLM followed by Awe treatment reduced bleomycin-induced weight loss, increased proline, glucose, and glycerid rates in Balf and which are characterized by their anti-inflammatory effect. In vivo, our data demonstrated that Awe administration protected against BLM-induced fibrosis as evidenced by TGFβ immunostaining increase in lungs fibrocytes as well as inflammatory infiltrate and alveoli. We also showed that acute bleomycin induced fibrosis was accompanied by an oxidative stress status in lung tissue as assessed by the increase of lipid peroxidation as well as antioxidant enzyme activities depletion such as superoxide dismutase (SOD) and catalase (CAT). More importantly, Awe treatment reversed all BLM-induced oxidative stress parameters disturbances. These findings provide an insight into the preventive and therapeutic potential of Awe treatment in the treatment of PF.
Abstract: This study aimed to investigate the antiulcer and antioxidant activities of myrtle berry seed aqueous extract (MBSAE) in a peptic ulcer model induced by ethanol in male Wistar rats. MBSAE is rich in total polyphenols, total flavonoids, and unsaturated fatty acids, particularly linoleic (18:2) and oleic (18:1) acids. MBSAE also exhibited in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 172.1 μg/mL) and superoxide anion (IC50 = 200.24 μg/mL) scavenging activities. In vivo, MBSAE provided dose-dependent protection against ethanol-induced gastric and duodenal macroscopic and histological alterations. Also, it inhibited secretory profile disturbances and lipid peroxidation, and preserved normal antioxidant enzyme activities and nonenzymatic antioxidant levels. More importantly, we showed that acute alcohol intoxication increased gastric and duodenal calcium, hydrogen peroxide, and free iron levels, whereas MBSAE treatment protected against intracellular mediator deregulation. In conclusion, we suggest that MBSAE has potent protective effects against alcohol-induced peptic ulcer in rat. This protection might be related in part to its antioxidant properties as well as its opposite effects on some studied intracellular mediators.
Pub.: 31 Jan '17, Pinned: 10 Aug '17
Abstract: We aimed in the present study to investigate the protective effect of Pistacia lentiscus oil against bleomycin-induced lung fibrosis as well as the involvement of oxidative stress in such protection. In this respect, adult male Wistar rats were used and divided into three groups of twenty each: control (NaCl, 0.9%), bleomycin, and bleomycin (4 mg/kg b.w.) + P. lentiscus oil (3 g/kg, b.w.). Animals were pretreated for 30 days before the induction of fibrosis by bleomycin and 1 wk after the induction of fibrosis. The oil principal compounds detected by gas chromatography analysis are: Linoleic and palmitic acids (70.6 and 24.7%, respectively). Our data demonstrated that P. lentiscus oil protected against bleomycin-induced fibrosis as evidenced by TGFβ immunostaining increase in lungs fibrocytes as well as inflammatory infiltrate. We also showed that acute bleomycin-induced fibrosis was accompanied by an oxidative stress in lung tissue as assessed by an increase of lipid peroxidation as well as antioxidant enzyme activities depletion such as superoxide dismutase (SOD) and catalase (CAT). More importantly, P. lentiscus oil treatment reversed all bleomycin-induced oxidative stress parameters disturbances. In conclusion, we suggest that P. lentiscus oil had potent protective effects against bleomycin-induced fibrosis due in part to its antioxidant properties.
Pub.: 14 Mar '17, Pinned: 10 Aug '17