Quantcast


CURATOR
A pinboard by
michael ajen

PhD student, University of Calabar, Nigeria.

PINBOARD SUMMARY

Environmental and occupational exposure to cadmium has been reported to have an effect on the kidney. This present study was conducted to investigate the ameliorative effect of Emilia sonchifolia on cadmium induced renal damage and to suggest the use of the plant extract for the treatment of various ailments associated with cadmium exposure and in a long run developed safe drugs from the plant extract. In the course of the experiment, various parameters like serum electrolytes, full blood count, enzymes activity of the kidneys, kidney histology and morphology were investigated to determine the effect of the plant extract on renal damage resulting from cadmium exposure .

3 ITEMS PINNED

Cadmium Chloride Induces DNA Damage and Apoptosis of Human Liver Carcinoma Cells via Oxidative Stress.

Abstract: Cadmium is a heavy metal that has been shown to cause its toxicity in humans and animals. Many documented studies have shown that cadmium produces various genotoxic effects such as DNA damage and chromosomal aberrations. Ailments such as bone disease, renal damage, and several forms of cancer are attributed to overexposure to cadmium. Although there have been numerous studies examining the effects of cadmium in animal models and a few case studies involving communities where cadmium contamination has occurred, its molecular mechanisms of action are not fully elucidated. In this research, we hypothesized that oxidative stress plays a key role in cadmium chloride-induced toxicity, DNA damage, and apoptosis of human liver carcinoma (HepG₂) cells. To test our hypothesis, cell viability was determined by MTT assay. Lipid hydroperoxide content stress was estimated by lipid peroxidation assay. Genotoxic damage was tested by the means of alkaline single cell gel electrophoresis (Comet) assay. Cell apoptosis was measured by flow cytometry assessment (Annexin-V/PI assay). The result of MTT assay indicated that cadmium chloride induces toxicity to HepG₂ cells in a concentration-dependent manner, showing a 48 hr-LD50 of 3.6 µg/mL. Data generated from lipid peroxidation assay resulted in a significant (p < 0.05) increase of hydroperoxide production, specifically at the highest concentration tested. Data obtained from the Comet assay indicated that cadmium chloride causes DNA damage in HepG₂ cells in a concentration-dependent manner. A strong concentration-response relationship (p < 0.05) was recorded between annexin V positive cells and cadmium chloride exposure. In summary, these in vitro studies provide clear evidence that cadmium chloride induces oxidative stress, DNA damage, and programmed cell death in human liver carcinoma (HepG₂) cells.

Pub.: 06 Jan '16, Pinned: 01 Sep '17