We describe the rotavirus genotypes before and after rotavirus vaccine introduction in Brazil. 86 studies reported 6,884 (15.2%) rotavirus-episodes among 45,305 children. Rotavirus caused 22.4% and 11.6% of cases before and after vaccine introduction. G1P and G9P, and G2P and heterotypic-strains were most common before and after vaccine introduction. The vaccines may have selected heterotypic strains in this highly-vaccinated population.