The genome of the kinetoplastid parasite, Leishmania major.
Research paper by
Alasdair C AC Ivens, Christopher S CS Peacock, Elizabeth A EA Worthey, Lee L Murphy, Gautam G Aggarwal, Matthew M Berriman, Ellen E Sisk, Marie-Adele MA Rajandream, Ellen E Adlem, Rita R Aert, Atashi A Anupama, Zina Z Apostolou, Philip P Attipoe, Nathalie N Bason, Christopher C Bauser, Alfred A Beck, Stephen M SM Beverley, Gabriella G Bianchettin, Katja K Borzym, Gordana G Bothe, Carlo V CV Bruschi, Matt M Collins, Eithon E Cadag, Laura L Ciarloni, Christine C Clayton, Richard M R RM Coulson, Ann A Cronin, Angela K AK Cruz, Robert M RM Davies, Javier J De Gaudenzi, Deborah E DE Dobson, Andreas A Duesterhoeft, Gholam G Fazelina, Nigel N Fosker, Alberto Carlos AC Frasch, Audrey A Fraser, Monika M Fuchs, Claudia C Gabel, Arlette A Goble, André A Goffeau, David D Harris, Christiane C Hertz-Fowler, Helmut H Hilbert, David D Horn, Yiting Y Huang, Sven S Klages, Andrew A Knights, Michael M Kube, Natasha N Larke, Lyudmila L Litvin, Angela A Lord, Tin T Louie, Marco M Marra, David D Masuy, K...
Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.