Indexed on: 01 Dec '66Published on: 01 Dec '66Published in: Histochemie. Histochemistry. Histochimie
A method is described for the histochemical determination of aldehyde dehydrogenase. The incubation medium contains: phosphate buffer, 0.06 m, pH 7.0, 8.0 ml; NaN3 in 0.06 m phosphate buffer, pH 7.0, 0.1 m, 1.0 ml; acetaldehyde, 1.0 m, 1.0 ml; Nitro-BT 2.5 mg; MgCl2·6H2O, 10 mg; NAD, 11 mg. Tris is not suitable as a buffer as the amino group of the buffer substance combines with the carbonyl group of the acetaldehyde to form azomethine. The consequence is a change in the pH value and a decrease in the concentration of the substrate. KCN should not be used to block the respiratory chain as it also influences the concentration of the substrate through the formation of cyanhydrin.