Yeast two-hybrid approaches to dissecting the plant defense response.

Research paper by Mawsheng M Chern, Todd T Richter, Pamela C PC Ronald

Indexed on: 19 Dec '06Published on: 19 Dec '06Published in: Methods in molecular biology (Clifton, N.J.)


We describe a reliable GAL4-based yeast two-hybrid system for identifying and isolating clones encoding proteins interacting with a protein of interest. This two-hybrid system gives extremely low background and few false-positive clones, making it ideal for library screening purposes. We have successfully used it not only to isolate Arabidopsis NPR1-interactors from rice but also to pull out the rice NPR1 ortholog using one of the interactors as bait.