Visible and fluorescent staining of two-dimensional gels.

Research paper by François F Chevalier, Valérie V Rofidal, Michel M Rossignol

Indexed on: 10 Nov '06Published on: 10 Nov '06Published in: Methods in molecular biology (Clifton, N.J.)


Staining of two-dimensional gel constitutes a crucial step in comparative proteome analysis with respect to the number of proteins analyzed, the accuracy of spot quantification, and compatibility with mass spectrometry. The present chapter describes procedures for several visible and fluorescent dyes compatible with mass spectrometry: colloidal Coomassie blue, silver nitrate, Sypro Ruby, Deep Purple, and 5-hexadecanoylamino-fluorescein.