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Viability and membrane lipid composition under a 57mT static magnetic field in Salmonella Hadar.

Research paper by Ramla R Ben Mouhoub, Alya A El May, Imen I Boujezza, Mohamed Marouen MM Sethom, Moncef M Feki, Ahmed A Landoulsi

Indexed on: 09 Apr '18Published on: 09 Apr '18Published in: Bioelectrochemistry



Abstract

The aim of this work is to demonstrate the effects of a static magnetic field (SMF) with an induction 12 equal to 57mT on the viability and membrane lipid composition of Salmonella Hadar. Results showed an increase in the viability of exposed bacteria compared to controls after 9h of exposure. Analysis with gas chromatography of total lipids (TLs) and different fractions of phospholipids: phosphatidylglycerols (PGs), phosphatidylethanolamines (PEs), and cardiolipins (CLs), separated by thin layer chromatography revealed changes in fatty acid levels during exposure. For TLs, the unsaturated fatty acids/saturated fatty acids ratio (UFAs/SFAs) had significantly increased after 9 h of exposure. The variation of this ratio seems to be essentially due to the increase of the proportion of unsaturated fatty acids with 18 carbons, in particular C. The analyses of fatty acid composition carried out on the scale of each fraction of phospholipids showed that CLs contributed significantly to the increase of the proportion of the unsaturated fatty acids between 6 and 9h of exposure thanks to their unsaturated chains with 18 carbons (especially C). CLs appear to be the main phospholipid involved in the adaptation of S. Hadar membranes to the SMF. Copyright © 2018. Published by Elsevier B.V.