Validated RP–LC Method for Standardization of Ashokarishta: A Polyherbal Formulation

Research paper by R. Govindarajan, Dhirendra P. Singh, Ajay K. S. Rawat

Indexed on: 16 Sep '08Published on: 16 Sep '08Published in: Chromatographia


A rapid LC-photodiode array method for the separation and identification of secondary plant metabolites especially phenolic compounds belonging to different types in a run (35 min) has been developed. The method has been optimized and validated using the selectivity, precision, recovery and robustness parameters with an aim for standardization of herbal drugs. Almost all the compounds have linearity with an R2 more than 98% and an RSD of less than 5% (in terms of variation of retention time). Inter-day and intra-day variability was also found to be less than 5%. The method thus developed has been successfully applied in identification and quantification of phenolic compounds present in polyherbal ayurvedic formulation (Ashokarishta). The results indicate that the method developed is rapid, accurate and robust for the analysis of different classes of phenolic compounds and can be successfully applied in the quality control and standardization of herbal drugs as well as polyherbal formulations.