Use of carboxypeptidase Y for carboxy-terminal sequence determination in proteins

Research paper by B. Martin, I. Svendsen, M. Ottesen

Indexed on: 01 Mar '77Published on: 01 Mar '77Published in: Carlsberg research communications


Treatment of several proteins-native pancreatic ribonuclease, human carbonic anhydrase B and human carbonic anhydrase C- with carboxypeptidase Y in the presence of 0.5% sodium dodecyl sulphate resulted in the sequential release of amino acid residues from the carboxyl terminus of the polypeptides. This provides a convenient method to study the carboxy-terminal sequence of proteins whose carboxyl termini are inaccessible in the native state to cleavage by carboxypeptidase.