Indexed on: 01 Jul '71Published on: 01 Jul '71Published in: Klinische Wochenschrift
A method is described for maximal B-cell stimulation of the human pancreas. The technique was tested in healthy volunteers. 100 g glucose were given orally 30 minutes before the combined injection of 0.5 g tolbutamide (Hoechst) and 1 mg crystalline glucagon (Lilly). The highest mean immunoreactive insulin concentration obtained was 829 mU/ml (method of Yalow and Berson). This is an appropriate method to measure the secretory capacity of the B-cells after intensive stimulation.