Indexed on: 20 Sep '17Published on: 20 Sep '17Published in: Microbial Pathogenesis
We aimed in this work to evaluate the effect of static magnetic field 200 mT (SMF) on the expression of genes involved in the biosynthetic pathway of cardiolipin: g3pd, plsB, cdsA, pgsA, pgpA, cls and phosphatidylethanolamine: pssA and psd in Salmonella enterica subsp enterica serovar Hadar. Bacteria were exposed to a SMF during 3, 6 and 9 h. RNA extraction was followed by Reverse Transcriptase Polymerase Chain Reaction RT-PCR. The relative quantification of mRNA expression levels using 16S rRNA doesn't change during the time exposure. RT-PCR was done for two exposure experiments. The gene expression using RT-PCR present no significant difference in case of plsB, cdsA, pgpA, pgsA and psd genes during the different exposure times. However, a significant increase was observed in the expression of g3pd and pssA genes after 6 h and for cls gene after 3 h of exposure, but any variation was notified after 9 h of exposure. So we can conclude from this study that cls, g3pd and pssA genes are required in the adaptation of Salmonella Hadar to SMF.