Ultrasensitive microfluidic paper-based electrochemical biosensor based on molecularly imprinted film and boronate affinity sandwich assay for glycoprotein detection.

Research paper by Xiaolu X Sun, Yannan Y Jian, He H Wang, Shenguang S Ge, Mei M Yan, Jinghua J Yu

Indexed on: 01 May '19Published on: 21 Mar '19Published in: ACS Applied Materials & Interfaces


In this work, we proposed a strategy that combined molecularly imprinted polymers (MIPs) and hybridization chain reaction into microfluidic paper-based analytical devices for ultrasensitive detecting target glycoprotein ovalbumin (OVA). In the fabrication, Au nanorods with large surface area and superior conductibility grew on paper cellulosic fiber as a matrix to fix a boronate-affinity sandwich assay. The composite of MIPs including 4-mercaptophenylboronic acid (MPBA) was able to capture target glycoprotein OVA. SiO2@Au nanocomposites labeled with MPBA and cerium dioxide (CeO2) modified nicked DNA double-strand polymers (SiO2@Au/dsDNA/CeO2) as signal tag was captured into the surface of electrode in the presence of OVA. Electrochemical signal generated by using nanoceria as redox active catalytic amplifiers in the presence of 1-naphthol in electrochemical assays. As a result, the electrochemical assay was fabricated and could be applied in the detection of OVA in the wide linear range of 1 pg/mL to 1000 ng/mL with a relatively low detection limit of 0.87 pg/mL (S/N=3). The results indicated that the proposed platform possessed potential applications in clinical diagnosis and other related field.