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Trypanosome microtubule-associated protein p15 as a vaccine for the prevention of African sleeping sickness.

Research paper by Reuven R Rasooly, Naomi N Balaban

Indexed on: 27 May '04Published on: 27 May '04Published in: Vaccine



Abstract

Trypanosomes cause African sleeping sickness, affecting millions of humans and animals. We tested trypanosome microtubule-associate protein (MAP p15) as a vaccine in mice, and show that p15 (native or recombinant) generated up to 100% protection from an otherwise lethal challenge of a heterologous strain of Trypanosoma brucei. We also tested the adenovirus as a vaccine delivery system and show that both adenoviral vector containing p15 gene or control adenovirus containing lacZ gene generated a protective response and exhibited strong CD8+ T-cell proliferation. These results suggest that the p15 protein itself is an effective vaccine and that the adenovirus may be used to mount a non-specific cellular immune response.