Indexed on: 01 Aug '19Published on: 31 Jul '19Published in: Nanotechnology
Zinc-based nanoparticles are promising material for various applications, including biomedicine. The aim of our study was to determine the effect of fluorescent europium-doped zinc oxide nanoparticles (ZnO:Eu NPs) on sperm parameters, cell apoptosis and integrity of blood-testis barrier (BTB) in mice. Nanostructures were orally administrated (IG) to adult mice (n=34). Animals were sacrificed after 3h, 24h, 7d and 14d following IG. Collected testes were quantitatively analysed for accumulation of ZnO:Eu NPs. Sperm was collected and analysed for viability and kinetic parameters. Microscopic evaluation based on immunofluorescence and histopathology were also conducted. Results showed, that ZnO:Eu NPs were able to overcome the BTB with their subsequent accumulation in testes. We did not observe neither toxic, nor pro-apoptotic effects of NPs on male reproductive system. Results suggested that ZnO:Eu NPs were able to accumulate in testes with no negative impact on sperm parameters, tissue architecture nor the integrity of BTB were observed. © 2019 IOP Publishing Ltd.