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Transfer of orally administrated ZnO:Eu nanoparticles through the blood-testis barrier, the effect on kinetic sperm parameters and apoptosis in mice testes.

Research paper by Paula P Kiełbik, Jaroslaw J Kaszewski, Sebastian S Dabrowski, Ricardo R Faundez, Bartlomiej Slawomir BS Witkowski, Lukasz L Wachnicki, Y Y Zhydachevskii, Rafal R Sapierzynski, Zdzislaw Z Gajewski, Marek M Godlewski, Michal M MM Godlewski

Indexed on: 01 Aug '19Published on: 31 Jul '19Published in: Nanotechnology



Abstract

Zinc-based nanoparticles are promising material for various applications, including biomedicine. The aim of our study was to determine the effect of fluorescent europium-doped zinc oxide nanoparticles (ZnO:Eu NPs) on sperm parameters, cell apoptosis and integrity of blood-testis barrier (BTB) in mice. Nanostructures were orally administrated (IG) to adult mice (n=34). Animals were sacrificed after 3h, 24h, 7d and 14d following IG. Collected testes were quantitatively analysed for accumulation of ZnO:Eu NPs. Sperm was collected and analysed for viability and kinetic parameters. Microscopic evaluation based on immunofluorescence and histopathology were also conducted. Results showed, that ZnO:Eu NPs were able to overcome the BTB with their subsequent accumulation in testes. We did not observe neither toxic, nor pro-apoptotic effects of NPs on male reproductive system. Results suggested that ZnO:Eu NPs were able to accumulate in testes with no negative impact on sperm parameters, tissue architecture nor the integrity of BTB were observed. © 2019 IOP Publishing Ltd.