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Tracheal Cartilage Isolation and Decellularization.

Research paper by Zachary Z Galliger, Angela A Panoskaltsis-Mortari

Indexed on: 22 Jul '17Published on: 22 Jul '17Published in: Methods in molecular biology (Clifton, N.J.)



Abstract

Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.