Time course of prion seeding activity in cerebrospinal fluid of scrapie-infected hamsters after intratongue and intracerebral inoculations.

Research paper by Christina D CD Orrù, Andrew G AG Hughson, Brent B Race, Gregory J GJ Raymond, Byron B Caughey

Indexed on: 13 Jan '12Published on: 13 Jan '12Published in: Journal of clinical microbiology


To assess prospects for early diagnosis of prion disease based on prion seeding activity in cerebrospinal fluid (CSF), we measured the activity over time in scrapie-infected hamsters by real-time quaking-induced conversion (RT-QuIC). After intracerebral inoculation, activity appeared in CSF within 1 day and plateaued weeks before the onset of clinical signs. However, after intratongue inoculation, activity first appeared in CSF with the onset of clinical signs, well after higher-level accumulation of seeding activity in brain.

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