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Tiba inhibition of in vitro organogenesis in excised tobacco leaf explants

Research paper by Harbinder S. Dhaliwal, Edward C. Yeung, Trevor A. Thorpe

Indexed on: 01 Mar '04Published on: 01 Mar '04Published in: In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association



Abstract

Triiodobenzoic acid (TIBA), an anti-auxin, was found to inhibit both shoot and root formation in cultured excised leaf explants of tobacco (Nicotiana tabacum L.). The shoot formation (SF) medium used required only exogenous cytokinin (N6-benzyladenine) and the root formation (RF) medium required both auxin (indole-3-butyric acid) and cytokinin (kinetin). By transferring the explants from SF or RF media to SF or RF media with TIBA (4.0×10−5M), respectively or vice versa, at different times in culture, it was found that TIBA inhibition was at the time of meristemoid formation and after determination of organogenesis. This indicates that TIBA interfered with endogenous auxin involvement in organized cell division.