Thionin functionalized signal amplification label derived dual-mode electrochemical immunoassay for sensitive detection of cardiac troponin I.

Research paper by Hui H Lv, Yueyun Y Li, Xiaobo X Zhang, Xinjin X Li, Zhen Z Xu, Lei L Chen, Degang D Li, Yunhui Y Dong

Indexed on: 17 May '19Published on: 26 Mar '19Published in: Biosensors and Bioelectronics


A sensitive sandwich-type electrochemical immunosensor was established by employing Au@Pt core-shell multi-branched nanoparticles, and thionin functionalized nitrogen/sulfur co-doped graphene oxide (N/S-cGO/L-lys/Au@Pt MBs/Thi) as a double signal label to detect cardiac troponin I (cTnI). In this work, Au nanorods functionalized polydopamine (Au NR@PDA) with high adsorption capacity and superior electroconductivity can provide an efficient substrate for immobilizing primary antibodies (Ab). In the proposed N/S-cGO/L-lys/Au@Pt MBs/Thi, an electrochemically active molecule, Thi was covalently bonded in the N/S-cGO/L-lys/Au@Pt MBs. It presented a strong differential pulse voltammetry (DPV) current signal without electron transfer mediators, and showed a high electrocatalytic activity toward HO reduction by using amperometric i-t (i-t). Impressively, with the synergistic effect of N/S-cGO/L-lys/Au@Pt MBs/Thi and Au NR@PDA, the developed dual-mode electrochemical immunosensor for cTnI detection showed a wide linear concentration range (50 fg/mL to 250 ng/mL, 750 fg/mL to 100 ng/mL) and a low detection limit (16.7 fg/mL, 250 fg/mL) via i-t and DPV, respectively. Furthermore, this immunosensor exhibited acceptable reproducibility, high sensitivity and good stability under optimal conditions. More importantly, the satisfactory results were obtained in detection of cTnI-spiked human serum samples, and the presented method may be a promising application in clinical bioanalysis. Copyright © 2019 Elsevier B.V. All rights reserved.