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The role of duplex stability for wavelength-shifting fluorescent DNA probes: energy transfer vs. exciton interactions in DNA "traffic lights".

Research paper by Sebastian S Barrois, Samantha S Wörner, Hans-Achim HA Wagenknecht

Indexed on: 09 Jul '14Published on: 09 Jul '14Published in: Photochemical & Photobiological Sciences



Abstract

Exciton interactions between thiazole orange and thiazole red as nucleotide substitutes in DNA hairpins interfere with efficient energy transfer and fluorescence color change as readout. This interference can be tuned by two structural parameters that control the hairpin duplex stability.