The method of eliminating phenol with the use of tyrosinase Agaricus bisporus immobilized in alginate

Research paper by I. I. Romanovskaya, Yu. A. Shesterenko, O. V. Sevast’yanov, I. E. Brusilovskii

Indexed on: 14 Mar '10Published on: 14 Mar '10Published in: Journal of Water Chemistry and Technology


We have considered the method of oxidizing aqueous solutions of phenol (0.25–2.00 mmol/dm3) with the use of a biocatalyst—a partially purified tyrosinase immobilized into calcium alginate. In optimal conditions (pH 7.2, T—25°C, transformation time—one hour, phenoloxidase activity—25–200 unit/cm3) when adding a PEG-6000 stabilizer the preparation stabilized complete oxidation of phenol in a periodic-action reactor during 12 cycles and retained a high degree of transformation of the substrate during the following 16 cycles. The concentrations of coagulants necessary for elimination of transformation products using the immobilized enzyme were reduced 2.5-fold compared with such for a free biocatalyst.