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The influence of 2,4,6-tribromophenyl isocyanate on the cellular and nuclear uptake of the SV 40 T antigen nuclear localization sequence.

Research paper by Stefan S Heckl, Alexander A Sturzu, Alireza A Gharabaghi, Hartmut H Echner, Thomas T Nagele

Indexed on: 12 Feb '08Published on: 12 Feb '08Published in: European Journal of Pharmacology



Abstract

The objective of the present study was to evaluate the influence of 2,4,6-tribromophenyl isocyanate (TBPI) on the cellular and nuclear uptake of the fluorescein isothiocyanate (FITC) labeled SV 40 T antigen nuclear localization sequence in human LN18 and U373 glioma cells. Therefore, the FITC-labeled nuclear localization sequence (NLS) of the SV 40 T antigen was coupled to 2,4,6-TBPI. This TBPI-NLS conjugate was taken up by the cell nuclei of more than 90% of human malignant glioma cells. The nuclearly stained cells showed clear signs of cell death. However only up to 10% of the cells were stained after incubation with the TBPI-lacking NLS of the SV 40 T antigen together with free, unbound TBPI. These cells stayed alive. TBPI, when bound to small peptides, may be an important component for future drugs against gliomas.