The great importance of normalization of LC-MS data for highly-accurate non-targeted metabolomics.

Research paper by Hajime H Mizuno, Kazuki K Ueda, Yuta Y Kobayashi, Naohiro N Tsuyama, Kenichiro K Todoroki, Jun Zhe JZ Min, Toshimasa T Toyo'oka

Indexed on: 09 Oct '16Published on: 09 Oct '16Published in: Biomedical Chromatography


The non-targeted metabolomics analysis of biological samples is very important in order to understand biological functions and diseases. Liquid chromatography combined with electrospray ionization-based mass spectrometry (LC-ESI-MS) has been a powerful tool and widely used for metabolomic analyses. However, the ionization efficiency of ESI fluctuates for various unexpected reasons such as matrix effects and intra-day variations of the instrument performances. In order to remove these fluctuations, normalization methods have been developed. Such techniques include increasing the sensitivity, separating co-eluting components and normalizing the ionization efficiencies. Normalization techniques allow simultaneously correcting of the ionization efficiencies of the detected metabolite peaks and achieving quantitative non-targeted metabolomics. In this review paper, we focused on these normalization methods for non-targeted metabolomics by LC-MS.