The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis.

Research paper by Do-Hwan DH Lim, Chun-Taek CT Oh, Langho L Lee, Jae-Sang JS Hong, Su-Hyun SH Noh, Seungwoo S Hwang, Sungchan S Kim, Sung-Jun SJ Han, Young Sik YS Lee

Indexed on: 06 Sep '11Published on: 06 Sep '11Published in: FEBS Letters


Small non-coding RNAs regulate gene expression in a sequence-specific manner. In Drosophila, Dicer-2 (Dcr-2) functions in the biogenesis of endogenous small interfering RNAs (endo-siRNAs). We identified 21 distinct proteins that exhibited a ≥ 1.5-fold change as a consequence of loss of dcr-2 function. Most of these were metabolic genes implicated in stress resistance and aging. dcr-2 Mutants had reduced lifespan and were hypersensitive to oxidative, endoplasmic reticulum, starvation, and cold stresses. Furthermore, loss of dcr-2 function led to abnormal lipid and carbohydrate metabolism. Our results suggest roles for the endo-siRNA pathway in metabolic regulation and defense against stress and aging in Drosophila.