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Testis and somaticXrcc-1 DNA repair gene expression

Research paper by Christi A. Walter, Jianwei Lu, Mukesh Bhakta, Zi-Qiang Zhou, Larry H. Thompson, John R. McCarrey

Indexed on: 01 Nov '94Published on: 01 Nov '94Published in: Somatic cell and molecular genetics



Abstract

The humanXRCC1 gene has been shown to be involved in DNA strand-break repair using the Chinese hamster ovary cell mutant EM9. The purpose of this study was to characterize the expression ofXrcc-1 to determine if there is tissue-specific expression and to provide a baseline of information for future studies that may involve alteringXrcc-1 expression in mice. Normal young adult male testis and enriched populations of pachytene spermatocytes and round spermatids displayed significantly higher levels ofXrcc-1 expression than other mouse tissue, althoughXrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to male germ cells, which is a striking contrast to the levels of expression obtained in somatic tissues from the mouse. The relatively high levels of expression identified in male germ cells indicateXrcc-1 may have an important role in male germ cell physiology.