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Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells.

Research paper by Jinlu J Tang, Xiaoxiao X He, Yanli Y Lei, Hui H Shi, Qiuping Q Guo, Jianbo J Liu, Dinggeng D He, Lv'an L Yan, Kemin K Wang

Indexed on: 19 Oct '17Published on: 19 Oct '17Published in: Chemical Communications



Abstract

A label-free and general thermo-controlled split apta-PCR strategy was first developed for the sensitive and specific detection of cancer cells. By integrating the temperature-responsive function of split aptamers with PCR amplification, a facile fluorescence assay of liver cancer SMMC-7721 cells was successfully realized with the detection of as low as 100 cells.