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Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

Research paper by Abhishek Santra, Hai Yu, Nova Tasnima, Musleh M. Muthana, Yanhong Li, Jie Zeng, Nicholas J. Kenyon, Angelique Y. Louie, Xi Chen

Indexed on: 25 Jan '16Published on: 17 Dec '15Published in: Chemical Science



Abstract

O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens, which are fucosylated glycans, as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.

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