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System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample

Imported: 24 Feb '17 | Published: 06 Jan '04

Timothy Roy Hansen, Bradley Scott Thomas, John Joseph Bianco, Matthew P. Collis

USPTO - Utility Patents

Abstract

A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically responsive particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the magnetically responsive particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

These and other objects, advantages and novel features of the invention will be more readily appreciated from the following detailed description when read in conjunction with the accompanying drawings, in which:

FIG. 1 is a diagram of an example of a nucleic acid assay preparation system employing a nucleic acid molecule extractor according to an embodiment of the present invention;

FIG. 2 is a perspective view of the nucleic acid molecule extractor shown in FIG. 1;

FIG. 3 is a top view of the nucleic acid molecule extractor shown in FIG. 2;

FIG. 4 is a exploded perspective view of an example of a tube rack used with the nucleic acid molecule extractor shown in FIGS. 1-3;

FIG. 5 is a detailed view of an example of the shape of one of the openings in the tube rack shown in FIG. 4;

FIG. 6 is a cross-sectional view of the nucleic acid molecule extractor taken along lines

6

6 in FIG. 3;

FIG. 7 is a detailed view of the portion of the nucleic acid molecule extractor designated in FIG. 6;

FIG. 8 is a exploded perspective view showing an example of the relationship between the tube blocks, electromagnets and thermoelectric devices included in the nucleic acid molecule extractor shown in FIGS. 1-3,

6 and

7;

FIG. 9 is a side view of the electromagnet printed circuit board shown in FIG. 8;

FIG. 10 is diagrammatic view illustrating the relationship of the fixed side and sliding cam of the nucleic acid molecule extractor shown in FIGS. 1-3,

6 and

7 when the movable magnets are positioned as shown in FIGS. 6 and 7;

FIG. 11 is a diagrammatic view illustrating the relationship between the fixed side and sliding cam of the nucleic acid molecule extractor shown in FIGS. 1-3,

6 and

7 when the magnets are being moved in a downward direction away from the tubes;

FIG. 12 is a diagrammatic view illustrating the relationship between the fixed side and sliding cam of the nucleic acid module extractor shown in FIGS. 1-3,

6 and

7 when the movable magnets are positioned at the downward most position away from the tubes;

FIG. 13 is a flowchart illustrating an example of the sequence of operations performed by the preparation system and, in particular, the extractor shown in FIGS. 1-3,

6 and

7;

FIG. 14 is a perspective view of another example of the nucleic acid molecule extractor shown in FIG. 1;

FIG. 15 is a top view of the nucleic acid molecule extractor shown in FIG. 2;

FIG. 16 is a exploded perspective view of an example of a tube rack used with the nucleic acid molecule extractor shown in FIGS. 14 and 15;

FIG. 17 is a perspective view of an assembled tube rack shown in FIG. 16;

FIG. 18 is a top view of the tube rack as shown in FIGS. 16 and 17;

FIG. 19 is a side view of the tube rack as shown in FIGS. 16 and 17;

FIG. 20 is a side view of the extractor shown in FIGS. 14 and 15;

FIG. 21 is a cross-sectional view of the nucleic acid molecule extractor taken along lines

21

21 in FIG. 15; and

FIG. 22 is a detailed view of the portion of the nucleic acid molecule extractor designated in FIG.

21.

Claims

1. A system for manipulating magnetically responsive particles having nucleic acid molecules bound thereto and being in a solution contained in at least one tube, said system comprising:

2. A system as claimed in claim 1, wherein:

3. A system as claimed in claim 1, wherein:

4. A system as claimed in claim 1, wherein said magnet moving device comprises:

5. A system as claimed in claim 1, wherein said magnet moving device comprises:

6. A system as claimed in claim 5 further comprising:

7. A system as claimed in claim 1, wherein:

8. A system as claimed in claim 7 further comprising:

9. A system as claimed in claim 1, further comprising:

10. A system as claimed in claim 1, wherein:

11. A system as claimed in claim 1, further comprising:

12. A system as claimed in claim 11, wherein:

13. A system as claimed in claim 12, further comprising:

14. A system as claimed in claim 1, wherein:

15. A system for manipulating magnetically responsive particles having nucleic acid molecules bound thereto and being in a solution contained in at least one tube, said system comprising:

16. A system as claimed in claim 15, further comprising: