Superoxide scavenging activity of pirfenidone-iron complex.

Research paper by Yoshihiro Y Mitani, Keizo K Sato, Yosuke Y Muramoto, Tomohiro T Karakawa, Masataka M Kitamado, Tatsuya T Iwanaga, Tetsuji T Nabeshima, Kumiko K Maruyama, Kazuko K Nakagawa, Kazuhiko K Ishida, Kazumi K Sasamoto

Indexed on: 13 May '08Published on: 13 May '08Published in: Biochemical and Biophysical Research Communications


Pirfenidone (PFD) is focused on a new anti-fibrotic drug, which can minimize lung fibrosis etc. We evaluated the superoxide (O2*-) scavenging activities of PFD and the PFD-iron complex by electron spin resonance (ESR) spectroscopy, luminol-dependent chemiluminescence assay, and cytochrome c reduction assay. Firstly, we confirmed that the PFD-iron complex was formed by mixing iron chloride with threefold molar PFD, and the complex was stable in distilled water and ethanol. Secondary, the PFD-iron complex reduced the amount of O2*- produced by xanthine oxidase/hypoxanthine without inhibiting the enzyme activity. Thirdly, it also reduced the amount of O2*- released from phorbor ester-stimulated human neutrophils. PFD alone showed few such effects. These results suggest the possibility that the O2*- scavenging effect of the PFD-iron complex contributes to the anti-fibrotic action of PFD used for treating idiopathic pulmonary fibrosis.