Study of heparin in intestinal ischemia and reperfusion in rats: morphologic and functional evaluation.

Research paper by M M MM Ghadie, R R Miranda-Ferreira, N S A NS Taha, A S AS Maroso, R J Z RJ Moreti, M P MP Andraus, P P Zempulski, H P HP Monteiro, M J MJ Simões, D J DJ Fagundes, A A Caricati-Neto, M O MO Taha

Indexed on: 03 Oct '12Published on: 03 Oct '12Published in: Transplantation Proceedings


To study whether treatment with heparin (HEP) attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were treated with HEP (100 U/kg intravenously) or saline solution (SS) before I (60 min), which was produced by occlusion of the superior mesenteric artery, and R (120 min). After I or I/R, we mounted 2-cm jejunal segment in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl, using a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were similar in the I + HEP and the I/R + HEP groups, but reduced in the I + SS and the I/R + SS groups. The jejunal enteric nerves were damaged in the I + SS and the I/R + SS, but not in the I + HEP and the I/R + HEP cohorts. These results suggested that HEP attenuated intestinal dysfunction caused by I and I/R.

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