Indexed on: 01 May '81Published on: 01 May '81Published in: Cellular and Molecular Life Sciences
Glyceraldehyde-3-P dehydrogenase (E.C. 22.214.171.124) from rabbit muscle is rapidly inactivated by methylglyoxal following pseudo first-order kinetics. Substrate, as well as inorganic phosphate, affords protection, whereas NAD+ is ineffective. The arginine residue implicated in the reaction is probably the anion binding site of the phosphate group of the substrate.