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Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312.

Research paper by Anna A Turska-Szewczuk, Hubert H Pietras, Katarzyna A KA Duda, Alicja A Kozińska, Agnieszka A Pękala, Otto O Holst

Indexed on: 20 Jul '14Published on: 20 Jul '14Published in: Carbohydrate Research



Abstract

The O-specific polysaccharide (OPS) obtained by mild-acid degradation of the lipopolysaccharide from Aeromonas sobria strain Pt312 was studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, NOESY, 1H-detected 1H,13C HSQC, and HMBC experiments. The sequence of the sugar residues was determined using 1H,1H NOESY and 1H,13C HMBC experiments. It was found that the OPS was built up of disaccharide repeating units composed of GlcpNAc and non-stoichiometrically O-acetylated Rhap residues, and had the structure.