Quantcast

Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility.

Research paper by Sarina S Bense, Sebastian S Bruchmann, Anika A Steffen, Theresia E B TEB Stradal, Susanne S Häussler, Juliane J Düvel

Indexed on: 07 Apr '19Published on: 14 Mar '19Published in: Molecular Microbiology



Abstract

The c-di-GMP-binding effector protein FlgZ has been demonstrated to control motility in the opportunistic pathogen Pseudomonas aeruginosa and it was suggested that c-di-GMP-bound FlgZ impedes motility via its interaction with the MotCD stator. To further understand how motility is downregulated in P. aeruginosa and to elucidate the general control mechanisms operating during bacterial growth, we examined the spatiotemporal activity of FlgZ. We re-annotated the P. aeruginosa flgZ open reading frame and demonstrate that FlgZ-mediated downregulation of motility is fine-tuned via three independent mechanisms. First, we found that flgZ gene is transcribed independently from flgMN in stationary growth phase to increase FlgZ protein levels in the cell. Second, FlgZ localizes to the cell pole upon c-di-GMP binding and third, we describe that FimV, a cell pole anchor protein, is involved in increasing the polar localized c-di-GMP bound FlgZ to inhibit both, swimming and swarming motility. Our results shed light on the complex dynamics and spatiotemporal control of c-di-GMP dependent bacterial motility phenotypes and on how the polar anchor protein FimV, the motor brake FlgZ and the stator proteins function to repress flagella-driven swimming and swarming motility. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.