Single step purification of concanavalin A (Con A) and bio-sugar production from jack bean using glucosylated magnetic nano matrix

Research paper by Ho Myeong Kim, Eun Jin Cho, Hyeun-Jong Bae

Indexed on: 14 Mar '16Published on: 23 Feb '16Published in: Bioresource Technology


Jack bean (JB, Canavalia ensiformis) is the source of bio-based products, such as proteins and bio-sugars that contribute to modern molecular biology and biomedical research. In this study, the use of jack bean was evaluated as a source for concanavalin A (Con A) and bio-sugar production. A novel method for purifying Con A from JBs was successfully developed using a glucosylated magnetic nano matrix (GMNM) as a physical support, which facilitated easy separation and purification of Con A. In addition, the enzymatic conversion rate of 2% (w/v) Con A extracted residue to bio-sugar was 98.4%. Therefore, this new approach for the production of Con A and bio-sugar is potentially useful for obtaining bio-based products from jack bean.

Graphical abstract 10.1016/j.biortech.2016.02.068.jpg
Figure 10.1016/j.biortech.2016.02.068.0.jpg
Figure 10.1016/j.biortech.2016.02.068.1.jpg
Figure 10.1016/j.biortech.2016.02.068.2.jpg