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Single-molecule monitoring in living cells by use of fluorescence microscopy.

Research paper by Wangxi W Luo, Kangmin K He, Tie T Xia, Xiaohong X Fang

Indexed on: 25 Sep '12Published on: 25 Sep '12Published in: Analytical and Bioanalytical Chemistry



Abstract

Monitoring single molecules in living cells is becoming a powerful tool for study of the location, dynamics, and kinetics of individual biomolecules in real time. In recent decades, several optical imaging techniques, for example epi-fluorescence microscopy, total internal reflection fluorescence microscopy (TIRFM), confocal microscopy, quasi-TIRFM, and single-point edge excitation subdiffraction microscopy (SPEED), have been developed, and their capability of capturing single-molecule dynamics in living cells has been demonstrated. In this review, we briefly summarize recent advances in the use of these imaging techniques for monitoring single-molecules in living cells for a better understanding of important biological processes, and discuss future developments.