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Simple multiplex real-time PCR for rapid detection of common 16S rRNA methyltransferase genes.

Research paper by Xuejun X Guo, Belinda B BB Dillon, Andrew N AN Ginn, Agnieszka M AM Wiklendt, Sally R SR Partridge, Jonathan R JR Iredell

Indexed on: 12 Jul '14Published on: 12 Jul '14Published in: Diagnostic Microbiology and Infectious Disease



Abstract

We have developed a real-time multiplex PCR assay to detect the three most common 16S rRNA methyltransferase genes (armA, rmtB and rmtC), which encode problematic high-level resistance to all clinically-relevant aminoglycoside antibiotics. All results were consistent with published conventional PCR assays and these genes still appear rare in Australia.