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Siliplant1 (Slp1) protein precipitates silica in sorghum silica cells.

Research paper by Santosh S Kumar, Nurit N Adiram-Filiba, Shula S Blum, Javier Arturo JA Sanchez-Lopez, Oren O Tzfadia, Ayelet A Omid, Hanne H Volpin, Yael Y Heifetz, Gil G Goobes, Rivka R Elbaum

Indexed on: 03 Jun '20Published on: 03 Jun '20Published in: Journal of experimental botany



Abstract

Silicon is absorbed by plant roots as silicic acid. The acid moves with the transpiration stream to the shoot, and mineralizes as silica. In grasses, leaf epidermal cells called silica cells deposit silica in most of their volume by unknown mechanism. Using bioinformatics tools, we identified a previously uncharacterized protein in sorghum (Sorghum bicolor), which we named Siliplant1 (Slp1). Slp1 is a basic protein with seven repeat units rich in proline, lysine, and glutamic acid. We found Slp1 RNA in sorghum immature leaf and immature inflorescence. In leaves, transcription was highest just before the active silicification zone (ASZ). There, Slp1 was localized specifically to developing silica cells, packed inside vesicles and scattered throughout the cytoplasm or near the cell boundary. These vesicles fused with the membrane, releasing their content in the apoplastic space. A short peptide that is repeated five times in Slp1 precipitated silica in vitro at a biologically relevant silicic acid concentration. Transient overexpression of Slp1 in sorghum resulted in ectopic silica deposition in all leaf epidermal cell-types. Our results show that Slp1 precipitates silica in sorghum silica cells. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.