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Silencing of PD-L2/B7-DC by Topical Application of Small Interfering RNA Inhibits Elicitation of Contact Hypersensitivity

Research paper by Emi Furusawa, Tatsukuni Ohno, Shigenori Nagai, Taisei Noda, Takuya Komiyama, Katsunori Kobayashi, Hidetoshi Hamomato, Michiyo Miyashin, Hiroo Yokozeki, Miyuki Azuma

Indexed on: 12 Apr '19Published on: 09 Apr '19Published in: Journal of Investigative Dermatology



Abstract

PD-L2 is a ligand for the immune checkpoint receptor PD-1, however, its regulatory function is unclear. We previously reported that silencing of CD86 in cutaneous dendritic cells (DCs) by topical siRNA application inhibits the elicitation of contact hypersensitivity (CHS). Here we investigated the effects of topical PD-L2 siRNA application on allergic skin disease. PD-L2 was induced in DCs concurrently with the elevation of MHC class II and CD86 expression. Topical PD-L2 siRNA application inhibited elicitation of CHS by suppressing early proinflammatory cytokine expression and migration of hapten-carrying DCs into lymph nodes.